Hotspot

A3228_gp24

Genome · 954-2460 · 1507 bp

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Pi 0.143

MarkerSeek score 82.40

Primer available yes

Label A3228_gp24

Feature Metadata

label_name: A3228_gp24
feature_type: gene
region: Genome
length_bp: 1507
variable_sites: 315
pi: 0.143
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
A3228_gp24_p1	GCCAAGTGGATTAAGGCAGT	TTTCTGGATAAGAACGCCCG	50.0	50.0	58.2	58.0	2562-2713 bp	0.666667	72.4
A3228_gp24_p2	GCCAAGTGGATTAAGGCAGT	CGTAGGCGAAAATTCTCCCA	50.0	50.0	58.2	58.0	2485 bp	0.333333	51.9
A3228_gp24_p3	GCCAAGTGGATTAAGGCAGT	ACGTAGGCGAAAATTCTCCC	50.0	50.0	58.2	58.0	2486 bp	0.333333	51.9
A3228_gp24_p4	GCCAAGTGGATTAAGGCAGT	ATAATGGTAGATGCCCGGGA	50.0	50.0	58.2	58.0	2376 bp	0.333333	51.7
A3228_gp24_p5	CAGTGCATCATGGATGGGAT	CGTAGGCGAAAATTCTCCCA	50.0	50.0	57.7	58.0	2250 bp	0.333333	50.9