Hotspot

A3228_gp07

Genome · 17294-18169 · 876 bp

Back to Monotropa

Pi 0.225

MarkerSeek score 83.00

Primer available yes

Label A3228_gp07

Feature Metadata

label_name: A3228_gp07
feature_type: gene
region: Genome
length_bp: 876
variable_sites: 278
pi: 0.225
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
A3228_gp07_p1	AACTCACTATGCGAGGGGTA	GTGTCCACAAACAACCAAGC	50.0	50.0	57.8	58.1	1690-1974 bp	0.666667	72.5
A3228_gp07_p2	GGGGACTTGCCAAGGTAAAA	GTGTCCACAAACAACCAAGC	50.0	50.0	58.0	58.1	1347 bp	0.333333	52.7
A3228_gp07_p3	GTAAAAAGGGGGACTTGCCA	GTGTCCACAAACAACCAAGC	50.0	50.0	58.0	58.1	1355 bp	0.333333	52.7
A3228_gp07_p4	GGGGACTTGCCAAGGTAAAA	GCCAGATCTTTACCGCATCT	50.0	50.0	58.0	57.8	995 bp	0.333333	51.3
A3228_gp07_p5	GTAAAAAGGGGGACTTGCCA	GCCAGATCTTTACCGCATCT	50.0	50.0	58.0	57.8	1003 bp	0.333333	51.3