Hotspot

A3228_gp06

Genome · 19448-21352 · 1905 bp

Back to Monotropa

Pi 0.203

MarkerSeek score 82.40

Primer available yes

Label A3228_gp06

Feature Metadata

label_name: A3228_gp06
feature_type: gene
region: Genome
length_bp: 1905
variable_sites: 533
pi: 0.203
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
A3228_gp06_p1	TCTGGGCATGTTTGTTGTCA	ACAATCCCAGGGAGGCATAT	45.0	50.0	57.9	58.2	2701-2877 bp	0.666667	72.2
A3228_gp06_p2	AATTCTCGACCATGGACACG	ACAATCCCAGGGAGGCATAT	50.0	50.0	58.0	58.2	2990 bp	0.333333	52.0
A3228_gp06_p3	TCGGACTTGTCTGGTTTTGG	ACAATCCCAGGGAGGCATAT	50.0	50.0	58.0	58.2	2715 bp	0.333333	51.7
A3228_gp06_p4	CATGGACACGCTTGCAAAAT	ACAATCCCAGGGAGGCATAT	45.0	50.0	57.9	58.2	2980 bp	0.333333	51.5
A3228_gp06_p5	AATTCTCGACCATGGACACG	CCAGCGTAGACATATTGCCA	50.0	50.0	58.0	57.8	2896 bp	0.333333	51.3