Hotspot

A3228_gp05

Genome · 22894-23646 · 753 bp

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Pi 0.16

MarkerSeek score 79.40

Primer available yes

Label A3228_gp05

Feature Metadata

label_name: A3228_gp05
feature_type: gene
region: Genome
length_bp: 753
variable_sites: 175
pi: 0.16
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
A3228_gp05_p1	CAGGGTTGAACTGGGGAAAA	TCCGCTGCTTTAATCCACTC	50.0	50.0	57.9	58.0	1600-1603 bp	0.666667	72.1
A3228_gp05_p2	ATTCCGTCGGAACAGCTTTT	TCCGCTGCTTTAATCCACTC	45.0	50.0	58.1	58.0	1081-1096 bp	0.666667	69.7
A3228_gp05_p3	CAGGGTTGAACTGGGGAAAA	TTAAATATGCTCCGTGGGGC	50.0	50.0	57.9	58.0	1840 bp	0.333333	52.5
A3228_gp05_p4	AAGCGTTGTTTGTTTTCCGG	TCCGCTGCTTTAATCCACTC	45.0	50.0	58.1	58.0	1306 bp	0.333333	52.5
A3228_gp05_p5	AAGCGTTGTTTGTTTTCCGG	TTAAATATGCTCCGTGGGGC	45.0	50.0	58.1	58.0	1543 bp	0.333333	52.5