primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
A3228_gp24_p1	A3228_gp24	1	GCCAAGTGGATTAAGGCAGT	TTTCTGGATAAGAACGCCCG	20	20	50.0	50.0	58.2	58.0	0.2	0.0	0.179118	954	2460	2562-2713	0.666667	72.4
A3228_gp24_p2	A3228_gp24	2	GCCAAGTGGATTAAGGCAGT	CGTAGGCGAAAATTCTCCCA	20	20	50.0	50.0	58.2	58.0	0.2	0.0	0.179118	954	2460	2485-2485	0.333333	51.9
A3228_gp24_p3	A3228_gp24	3	GCCAAGTGGATTAAGGCAGT	ACGTAGGCGAAAATTCTCCC	20	20	50.0	50.0	58.2	58.0	0.2	0.0	0.180524	954	2460	2486-2486	0.333333	51.9
A3228_gp24_p4	A3228_gp24	4	GCCAAGTGGATTAAGGCAGT	ATAATGGTAGATGCCCGGGA	20	20	50.0	50.0	58.2	58.0	0.2	29.3	0.199439	954	2460	2376-2376	0.333333	51.7
A3228_gp24_p5	A3228_gp24	5	CAGTGCATCATGGATGGGAT	CGTAGGCGAAAATTCTCCCA	20	20	50.0	50.0	57.7	58.0	8.2	0.0	0.306519	954	2460	2250-2250	0.333333	50.9
A3228_gp21-A3228_gp20_p1	A3228_gp21-A3228_gp20	1	CGAATGGATCAGAAAGGGCA	TCGTCGATTGCTAGCTCCTA	20	20	50.0	50.0	58.0	58.0	0.0	17.2	0.079514	4137	5307	1903-1903	0.333333	52.7
A3228_gp21-A3228_gp20_p2	A3228_gp21-A3228_gp20	2	GGTTTGCACACCCAAAAGAC	TCGTCGATTGCTAGCTCCTA	20	20	50.0	50.0	58.1	58.0	11.2	17.2	0.102239	4137	5307	2381-2381	0.333333	52.5
A3228_gp21-A3228_gp20_p3	A3228_gp21-A3228_gp20	3	CGAATGGATCAGAAAGGGCA	AACCCATGCGTGCTTCTTTA	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.138399	4137	5307	1563-1563	0.333333	52.2
A3228_gp21-A3228_gp20_p4	A3228_gp21-A3228_gp20	4	CGAATGGATCAGAAAGGGCA	GGTTTAACAACGGCTACCCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.147805	4137	5307	1606-1606	0.333333	52.2
A3228_gp21-A3228_gp20_p5	A3228_gp21-A3228_gp20	5	CGAATGGATCAGAAAGGGCA	TGGTTTAACAACGGCTACCC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.147805	4137	5307	1607-1607	0.333333	52.2
A3228_gp12-A3228_gp11_p1	A3228_gp12-A3228_gp11	1	CGTATCCCCGGCTAATTCTG	TGGACGCAACCCCAAATTAA	20	20	55.0	45.0	57.9	58.0	0.0	0.0	0.083488	9856	10616	1006-1012	0.666667	71.2
A3228_gp12-A3228_gp11_p2	A3228_gp12-A3228_gp11	2	CGTATCCCCGGCTAATTCTG	TTTATGGACGCAACCCCAAA	20	20	55.0	45.0	57.9	58.0	0.0	0.0	0.083488	9856	10616	1010-1016	0.666667	71.2
A3228_gp12-A3228_gp11_p3	A3228_gp12-A3228_gp11	3	TGCGAGTGATCCATAAACGG	TGGACGCAACCCCAAATTAA	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.068613	9856	10616	1168-1174	0.666667	70.9
A3228_gp12-A3228_gp11_p4	A3228_gp12-A3228_gp11	4	TGCGAGTGATCCATAAACGG	TTTATGGACGCAACCCCAAA	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.068613	9856	10616	1172-1178	0.666667	70.9
A3228_gp12-A3228_gp11_p5	A3228_gp12-A3228_gp11	5	TGCGAGTGATCCATAAACGG	AAGGTTATGAGCCTTGCGAG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.095250	9856	10616	1374-1374	0.333333	52.6
A3228_gt05-A3228_gp09_p1	A3228_gt05-A3228_gp09	1	GTAGCTCGCAAGGCTCATAA	AGGCTTTTGTCCATCCCTTG	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.296200	11668	12231	1645-1645	0.333333	51.0
A3228_gt05-A3228_gp09_p2	A3228_gt05-A3228_gp09	2	TAGCTCGCAAGGCTCATAAC	AGGCTTTTGTCCATCCCTTG	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.296200	11668	12231	1644-1644	0.333333	51.0
A3228_gt05-A3228_gp09_p3	A3228_gt05-A3228_gp09	3	GTAGCTCGCAAGGCTCATAA	TGAAACGGGGCCCTCTATAA	20	20	50.0	50.0	57.8	58.1	0.0	24.4	0.342760	11668	12231	1241-1241	0.333333	50.6
A3228_gt05-A3228_gp09_p4	A3228_gt05-A3228_gp09	4	TAGCTCGCAAGGCTCATAAC	TGAAACGGGGCCCTCTATAA	20	20	50.0	50.0	57.8	58.1	0.0	24.4	0.342760	11668	12231	1240-1240	0.333333	50.6
A3228_gt05-A3228_gp09_p5	A3228_gt05-A3228_gp09	5	TAGCTCGCAAGGCTCATAAC	TTGAAACGGGGCCCTCTATA	20	20	50.0	50.0	57.8	58.1	0.0	24.4	0.342760	11668	12231	1241-1241	0.333333	50.6
A3228_gp09_p1	A3228_gp09	1	GGCTCAGGATTGCCCTTTTA	AATCCAGCTTTTCCTTGCCA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.157885	12232	12843	1396-1396	0.333333	52.1
A3228_gp09_p2	A3228_gp09	2	GGCTCAGGATTGCCCTTTTA	ACTAGATCCGGTTACCCCTG	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.227504	12232	12843	1672-1672	0.333333	51.5
A3228_gp09_p3	A3228_gp09	3	GGCTCAGGATTGCCCTTTTA	ATTCCTCGGTGGGGATTGTA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.256078	12232	12843	1534-1534	0.333333	51.3
A3228_gp09_p4	A3228_gp09	4	GGCTCAGGATTGCCCTTTTA	TATTCCTCGGTGGGGATTGT	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.256078	12232	12843	1535-1535	0.333333	51.3
A3228_gp09_p5	A3228_gp09	5	GGCTCAGGATTGCCCTTTTA	TAGCTCTCGCTCTTCCAGTT	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.303139	12232	12843	1589-1589	0.333333	50.9
A3228_gp09-A3228_gp08_p1	A3228_gp09-A3228_gp08	1	CAAGGGATGGACAAAAGCCT	TCCCTCTCTCTCCCCTTACT	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.074379	12844	13290	1000-1033	0.666667	69.3
A3228_gp09-A3228_gp08_p2	A3228_gp09-A3228_gp08	2	CAAGGGATGGACAAAAGCCT	AATCCAGCTTTTCCTTGCCA	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.085142	12844	13290	668-700	0.666667	69.1
A3228_gp09-A3228_gp08_p3	A3228_gp09-A3228_gp08	3	CAAGGGATGGACAAAAGCCT	GAGAGATGGCTGAGTGGTTG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.092487	12844	13290	1073-1106	0.666667	69.1
A3228_gp09-A3228_gp08_p4	A3228_gp09-A3228_gp08	4	CAAGGGATGGACAAAAGCCT	CCTCTCTCTCCCCTTACTCG	20	20	50.0	60.0	58.1	58.0	0.0	0.0	0.095472	12844	13290	998-1031	0.666667	69.1
A3228_gp09-A3228_gp08_p5	A3228_gp09-A3228_gp08	5	TATAGAGGGCCCCGTTTCAA	TCCCTCTCTCTCCCCTTACT	20	20	50.0	55.0	58.1	58.0	21.4	0.0	0.120939	12844	13290	1403-1436	0.666667	68.7
A3228_gt06-A3228_gr04_p1	A3228_gt06-A3228_gr04	1	AGTAAGGGGAGAGAGAGGGA	CCGTACGACTTGCATGTGTA	20	20	55.0	50.0	58.0	58.0	0.0	18.8	0.017913	13718	14575	626-1013	0.666667	73.5
A3228_gt06-A3228_gr04_p2	A3228_gt06-A3228_gr04	2	AGTAAGGGGAGAGAGAGGGA	TCGCATCCTCTGTTTTACCG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.007830	13718	14575	1444-1444	0.333333	53.3
A3228_gt06-A3228_gr04_p3	A3228_gt06-A3228_gr04	3	AGTAAGGGGAGAGAGAGGGA	CATTGCGGAAAATTCCCCAC	20	20	55.0	50.0	58.0	58.0	0.0	6.6	0.011999	13718	14575	1296-1296	0.333333	53.2
A3228_gt06-A3228_gr04_p4	A3228_gt06-A3228_gr04	4	AGTAAGGGGAGAGAGAGGGA	TGCCCCTACTATACTCCAGC	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.014369	13718	14575	1566-1566	0.333333	53.2
A3228_gt06-A3228_gr04_p5	A3228_gt06-A3228_gr04	5	AGTAAGGGGAGAGAGAGGGA	AAATTCCCTCTGCCCCTACT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.016168	13718	14575	1576-1576	0.333333	53.2
A3228_gr04_p1	A3228_gr04	1	AAAGTGAAGACCGGGCTTTT	AGCTTTGCATTGGAATCCCA	20	20	45.0	45.0	57.9	58.1	26.9	0.0	0.125988	14576	16049	2539-2539	0.333333	52.3
A3228_gr04_p2	A3228_gr04	2	AAGTGAAGACCGGGCTTTTT	AGCTTTGCATTGGAATCCCA	20	20	45.0	45.0	57.9	58.1	26.9	0.0	0.125988	14576	16049	2538-2538	0.333333	52.3
A3228_gr04_p3	A3228_gr04	3	AAGTGAAGACCGGGCTTTTT	AGAGAAACCCCATCCCTCTC	20	20	45.0	55.0	57.9	58.1	26.9	0.0	0.180943	14576	16049	2376-2376	0.333333	51.9
A3228_gr04_p4	A3228_gr04	4	AAGTGAAGACCGGGCTTTTT	GAGAAACCCCATCCCTCTCT	20	20	45.0	55.0	57.9	58.1	26.9	0.0	0.180943	14576	16049	2375-2375	0.333333	51.9
A3228_gr04_p5	A3228_gr04	5	AAGTGAAGACCGGGCTTTTT	GAGAGAAACCCCATCCCTCT	20	20	45.0	55.0	57.9	58.1	26.9	0.0	0.180943	14576	16049	2377-2377	0.333333	51.9
A3228_gp07_p1	A3228_gp07	1	AACTCACTATGCGAGGGGTA	GTGTCCACAAACAACCAAGC	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.233324	17294	18169	1690-1974	0.666667	72.5
A3228_gp07_p2	A3228_gp07	2	GGGGACTTGCCAAGGTAAAA	GTGTCCACAAACAACCAAGC	20	20	50.0	50.0	58.0	58.1	9.6	0.0	0.082693	17294	18169	1347-1347	0.333333	52.7
A3228_gp07_p3	A3228_gp07	3	GTAAAAAGGGGGACTTGCCA	GTGTCCACAAACAACCAAGC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.082693	17294	18169	1355-1355	0.333333	52.7
A3228_gp07_p4	A3228_gp07	4	GGGGACTTGCCAAGGTAAAA	GCCAGATCTTTACCGCATCT	20	20	50.0	50.0	58.0	57.8	9.6	0.0	0.250656	17294	18169	995-995	0.333333	51.3
A3228_gp07_p5	A3228_gp07	5	GTAAAAAGGGGGACTTGCCA	GCCAGATCTTTACCGCATCT	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.250656	17294	18169	1003-1003	0.333333	51.3
A3228_gp07-A3228_gp06_p1	A3228_gp07-A3228_gp06	1	CGGGAATCTATGCCTTGCTT	CCCATCGATATCTCCCTGGT	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.082951	18170	19447	1966-2017	0.666667	72.5
A3228_gp07-A3228_gp06_p2	A3228_gp07-A3228_gp06	2	GGGGATGAGGATGAGGATGA	CCCATCGATATCTCCCTGGT	20	20	55.0	55.0	58.0	58.1	0.0	0.0	0.091733	18170	19447	2339-2339	0.333333	52.6
A3228_gp07-A3228_gp06_p3	A3228_gp07-A3228_gp06	3	CGGGAATCTATGCCTTGCTT	CAGTTGATTCCTTGCACCCT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.111987	18170	19447	1958-1958	0.333333	52.4
A3228_gp07-A3228_gp06_p4	A3228_gp07-A3228_gp06	4	CGGGAATCTATGCCTTGCTT	TCAGTTGATTCCTTGCACCC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.117428	18170	19447	1959-1959	0.333333	52.4
A3228_gp07-A3228_gp06_p5	A3228_gp07-A3228_gp06	5	GGGGATGAGGATGAGGATGA	CAGTTGATTCCTTGCACCCT	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.120769	18170	19447	2280-2280	0.333333	52.4
A3228_gp06_p1	A3228_gp06	1	TCTGGGCATGTTTGTTGTCA	ACAATCCCAGGGAGGCATAT	20	20	45.0	50.0	57.9	58.2	0.0	15.4	0.225822	19448	21352	2701-2877	0.666667	72.2
A3228_gp06_p2	A3228_gp06	2	AATTCTCGACCATGGACACG	ACAATCCCAGGGAGGCATAT	20	20	50.0	50.0	58.0	58.2	21.5	15.4	0.171534	19448	21352	2990-2990	0.333333	52.0
A3228_gp06_p3	A3228_gp06	3	TCGGACTTGTCTGGTTTTGG	ACAATCCCAGGGAGGCATAT	20	20	50.0	50.0	58.0	58.2	9.4	15.4	0.199595	19448	21352	2715-2715	0.333333	51.7
A3228_gp06_p4	A3228_gp06	4	CATGGACACGCTTGCAAAAT	ACAATCCCAGGGAGGCATAT	20	20	45.0	50.0	57.9	58.2	0.0	15.4	0.230226	19448	21352	2980-2980	0.333333	51.5
A3228_gp06_p5	A3228_gp06	5	AATTCTCGACCATGGACACG	CCAGCGTAGACATATTGCCA	20	20	50.0	50.0	58.0	57.8	21.5	0.0	0.249087	19448	21352	2896-2896	0.333333	51.3
A3228_gp05_p1	A3228_gp05	1	CAGGGTTGAACTGGGGAAAA	TCCGCTGCTTTAATCCACTC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.104830	22894	23646	1600-1603	0.666667	72.1
A3228_gp05_p2	A3228_gp05	2	ATTCCGTCGGAACAGCTTTT	TCCGCTGCTTTAATCCACTC	20	20	45.0	50.0	58.1	58.0	28.4	0.0	0.131966	22894	23646	1081-1096	0.666667	69.7
A3228_gp05_p3	A3228_gp05	3	CAGGGTTGAACTGGGGAAAA	TTAAATATGCTCCGTGGGGC	20	20	50.0	50.0	57.9	58.0	0.0	3.1	0.104994	22894	23646	1840-1840	0.333333	52.5
A3228_gp05_p4	A3228_gp05	4	AAGCGTTGTTTGTTTTCCGG	TCCGCTGCTTTAATCCACTC	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.108223	22894	23646	1306-1306	0.333333	52.5
A3228_gp05_p5	A3228_gp05	5	AAGCGTTGTTTGTTTTCCGG	TTAAATATGCTCCGTGGGGC	20	20	45.0	50.0	58.1	58.0	0.0	3.1	0.108388	22894	23646	1543-1543	0.333333	52.5
A3228_gt18-A3228_gt19_p1	A3228_gt18-A3228_gt19	1	GTAGCTCGCAAGGCTCATAA	TAGGTTCGAGTCCGAGTAGC	20	20	50.0	55.0	57.8	58.3	0.0	3.6	0.563727	34574	34937	443-443	0.333333	48.8
A3228_gt18-A3228_gt19_p2	A3228_gt18-A3228_gt19	2	TAGCTCGCAAGGCTCATAAC	TAGGTTCGAGTCCGAGTAGC	20	20	50.0	55.0	57.8	58.3	0.0	3.6	0.563727	34574	34937	442-442	0.333333	48.8
A3228_gt18-A3228_gt19_p3	A3228_gt18-A3228_gt19	3	AGGTCATGGGTTCAAATCCC	TAGGTTCGAGTCCGAGTAGC	20	20	50.0	55.0	57.5	58.3	0.0	3.6	0.870908	34574	34937	418-418	0.333333	46.4
A3228_gt18-A3228_gt19_p4	A3228_gt18-A3228_gt19	4	CCTTGAGGTCATGGGTTCAA	TAGGTTCGAGTCCGAGTAGC	20	20	50.0	55.0	57.4	58.3	4.3	3.6	0.923494	34574	34937	423-423	0.333333	45.9
A3228_gt18-A3228_gt19_p5	A3228_gt18-A3228_gt19	5	TAGCTCGCAAGGCTCATAAC	ATCTAGGTTCGAGTCCGAGT	20	20	50.0	50.0	57.8	57.3	0.0	14.6	0.938931	34574	34937	445-445	0.333333	45.8