Hotspot

rpl20

LSC · 58651-59061 · 411 bp

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Pi 0.158

MarkerSeek score 77.90

Primer available yes

Label rpl20

Feature Metadata

label_name: rpl20
feature_type: gene
region: LSC
length_bp: 411
variable_sites: 63
pi: 0.158
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20_p1	AGAACGGACTAACAGGGTCA	AAACAGAAACACGACCGACA	50.0	45.0	58.0	58.0	1181 bp	0.5	59.9
rpl20_p2	AGAACGGACTAACAGGGTCA	AACAACAACCCGCAGATTCT	50.0	45.0	58.0	58.0	1117 bp	0.5	59.8
rpl20_p3	GTGCGACTCGTTCAGATCTT	AAACAGAAACACGACCGACA	50.0	45.0	58.0	58.0	1440 bp	0.5	59.8
rpl20_p4	TGCGACTCGTTCAGATCTTG	AAACAGAAACACGACCGACA	50.0	45.0	58.0	58.0	1439 bp	0.5	59.8
rpl20_p5	GTGCGACTCGTTCAGATCTT	AACAACAACCCGCAGATTCT	50.0	45.0	58.0	58.0	1376 bp	0.5	59.7