Hotspot

clpP

LSC · 56999-57718 · 720 bp

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Pi 0.179

MarkerSeek score 82.20

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 720
variable_sites: 125
pi: 0.179
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CGGTATAATCTTGGCTCGGG	TGACCCTGTTAGTCCGTTCT	55.0	50.0	57.9	58.0	1353 bp	0.5	59.3
clpP_p2	CGGTATAATCTTGGCTCGGG	AAGATCTGAACGAGTCGCAC	55.0	50.0	57.9	58.0	1094 bp	0.5	59.2
clpP_p3	CGGTATAATCTTGGCTCGGG	CAAGATCTGAACGAGTCGCA	55.0	50.0	57.9	58.0	1095 bp	0.5	59.2
clpP_p4	CGGTATAATCTTGGCTCGGG	CTGAACGAGTCGCACATACA	55.0	50.0	57.9	58.0	1089 bp	0.5	59.2
clpP_p5	CGGTATAATCTTGGCTCGGG	AGTTGTCCGCTTTCCTTTGT	55.0	45.0	57.9	57.9	1122 bp	0.5	58.9