Hotspot

trnI (CAU)-rpl23

IRa · 164899-165417 · 519 bp

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Pi 0.0938
MarkerSeek score 79.70
Primer available yes
Label trnI (CAU)-rpl23

Feature Metadata

label_name
trnI (CAU)-rpl23
feature_type
igs
region
IRa
length_bp
519
variable_sites
47
pi
0.0938
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI (CAU)-rpl23_p1 CCTGTTAAGCATCCATGGCT TCTTCTAGCCTTTCCCGGAA 50.0 50.0 57.9 58.1 914-1061 bp 1.0 86.4
trnI (CAU)-rpl23_p2 CCTGTTAAGCATCCATGGCT CGGTATAGACGCTTATGGCC 50.0 55.0 57.9 58.0 1198-1340 bp 1.0 85.5
trnI (CAU)-rpl23_p3 CCTGTTAAGCATCCATGGCT ACTGTCTACGGTTCCATTGC 50.0 50.0 57.9 57.9 1067-1209 bp 1.0 85.5
trnI (CAU)-rpl23_p4 CCTGTTAAGCATCCATGGCT TTATGGCCTCCCCCTCTATG 50.0 55.0 57.9 58.0 1186-1328 bp 1.0 85.3
trnI (CAU)-rpl23_p5 CCTGTTAAGCATCCATGGCT ACCACAAAGATGCTGTCCAG 50.0 50.0 57.9 58.1 1130 bp 0.5 58.7