Hotspot

rpl33-psaJ

LSC · 60046-60423 · 378 bp

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Pi 0.0563

MarkerSeek score 76.30

Primer available yes

Label rpl33-psaJ

Feature Metadata

label_name: rpl33-psaJ
feature_type: igs
region: LSC
length_bp: 378
variable_sites: 21
pi: 0.0563
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-psaJ_p1	ACACCCTTTCGAACACAAGT	ACATATCTTTCCGTGGCACC	45.0	50.0	57.6	58.0	566-599 bp	1.0	80.0
rpl33-psaJ_p2	ACACCCTTTCGAACACAAGT	TAAGTGCTCTATGGTTCGCG	45.0	50.0	57.6	57.8	541-574 bp	1.0	78.8
rpl33-psaJ_p3	ACACCCTTTCGAACACAAGT	GCTCTATGGTTCGCGTCTTT	45.0	50.0	57.6	58.4	536-569 bp	1.0	77.6
rpl33-psaJ_p4	ACACCCTTTCGAACACAAGT	CGCGTCTTTAGCAGGTCTAT	45.0	50.0	57.6	57.5	525-558 bp	1.0	76.6
rpl33-psaJ_p5	ACACCCTTTCGAACACAAGT	TCGCGTCTTTAGCAGGTCTA	45.0	50.0	57.6	58.5	526-559 bp	1.0	76.2