Hotspot

rps12-rpl20

LSC · 57904-58650 · 747 bp

Back to Monopsis

Pi 0.116

MarkerSeek score 79.60

Primer available yes

Label rps12-rpl20

Feature Metadata

label_name: rps12-rpl20
feature_type: igs
region: LSC
length_bp: 747
variable_sites: 86
pi: 0.116
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-rpl20_p1	GGAAGGCGTGATCCGTTTAT	AAACAGAAACACGACCGACA	50.0	45.0	58.1	58.0	2100 bp	0.5	59.5
rps12-rpl20_p2	TCGATCTGGGGTCTGTACTC	AAACAGAAACACGACCGACA	55.0	45.0	57.9	58.0	2030 bp	0.5	59.5
rps12-rpl20_p3	GGAAGGCGTGATCCGTTTAT	AACAACAACCCGCAGATTCT	50.0	45.0	58.1	58.0	2036 bp	0.5	59.4
rps12-rpl20_p4	TCGATCTGGGGTCTGTACTC	AACAACAACCCGCAGATTCT	55.0	45.0	57.9	58.0	1966 bp	0.5	59.4
rps12-rpl20_p5	GAAGCTTCACGATCGCATTG	AAACAGAAACACGACCGACA	50.0	45.0	57.9	58.0	1812 bp	0.5	59.2