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atpH-atpI

LSC · 6709-7771 · 1063 bp

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Pi 0.0461

MarkerSeek score 77.90

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1063
variable_sites: 49
pi: 0.0461
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AATAGAAGCAAGCCCGACAG	TAGGTGAATCCATGGAGGGG	50.0	55.0	58.0	58.2	1158-1170 bp	1.0	81.7
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	TAGGTGAATCCATGGAGGGG	45.0	55.0	58.0	58.2	1124-1136 bp	1.0	81.7
atpH-atpI_p3	AGCCAATCCAGCAGCAATAA	TAGGTGAATCCATGGAGGGG	45.0	55.0	57.9	58.2	1140-1152 bp	1.0	80.8
atpH-atpI_p4	GCGATACCTTCTACAGCCTG	TAGGTGAATCCATGGAGGGG	55.0	55.0	57.9	58.2	1211-1223 bp	1.0	80.6
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	TAGGTGAATCCATGGAGGGG	45.0	55.0	58.2	58.2	1125-1137 bp	1.0	80.6