Hotspot

rps8

Genome · 48656-49487 · 832 bp

Back to Monomorphina

Pi 0.317

MarkerSeek score 83.50

Primer available yes

Label rps8

Feature Metadata

label_name: rps8
feature_type: gene
region: Genome
length_bp: 832
variable_sites: 247
pi: 0.317
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8_p1	TATGAGTCCCGTGCTATCGT	ATTGCACGTGAATTGAGGGA	50.0	45.0	58.0	57.8	1829 bp	0.5	58.2
rps8_p2	TATGAGTCCCGTGCTATCGT	TTGCACGTGAATTGAGGGAT	50.0	45.0	58.0	57.8	1828 bp	0.5	58.2
rps8_p3	TATGAGTCCCGTGCTATCGT	GTTGTTCCACGTCTTCAGGA	50.0	50.0	58.0	57.8	1678 bp	0.5	57.9
rps8_p4	TATGAGTCCCGTGCTATCGT	AGTTGTTCCACGTCTTCAGG	50.0	50.0	58.0	57.8	1679 bp	0.5	57.9
rps8_p5	GATTCGAACCAGCGATGTCT	TTGCACGTGAATTGAGGGAT	50.0	45.0	58.1	57.8	1862 bp	0.5	57.8