Hotspot

rpl23

Genome · 57858-58576 · 719 bp

Back to Monomorphina

Pi 0.382

MarkerSeek score 83.90

Primer available yes

Label rpl23

Feature Metadata

label_name: rpl23
feature_type: gene
region: Genome
length_bp: 719
variable_sites: 261
pi: 0.382
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23_p1	ACACCTCGATGATTACGACC	CACTATGGCTTGTTGGGACA	50.0	50.0	57.1	57.8	1131 bp	0.5	51.5
rpl23_p2	ACACCTCGATGATTACGACC	TTCCACTATGGCTTGTTGGG	50.0	50.0	57.1	57.8	1134 bp	0.5	51.4
rpl23_p3	ACACCTCGATGATTACGACC	CAGGATTAGCTGCGGTTTCA	50.0	50.0	57.1	58.3	1107 bp	0.5	51.0
rpl23_p4	CACCTCGATGATTACGACCTC	CACTATGGCTTGTTGGGACA	52.4	50.0	58.0	57.8	1130 bp	0.5	50.4
rpl23_p5	ACACCTCGATGATTACGACC	AGTTCATGCTCTGGCGATTC	50.0	50.0	57.1	58.3	1518 bp	0.5	50.4