Hotspot

psbC

Genome · 4270-10497 · 6228 bp

Back to Monomorphina

Pi 0.294

MarkerSeek score 76.70

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: Genome
length_bp: 6228
variable_sites: 1751
pi: 0.294
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	AGGTTTAGTTTCGCTGAAGC	AGCCTTGAGAAACTTTGGCT	45.0	45.0	56.4	57.6	1351 bp	0.5	43.9
psbC_p2	AAGGTTTAGTTTCGCTGAAGC	AGCCTTGAGAAACTTTGGCT	42.9	45.0	57.1	57.6	1352 bp	0.5	41.8
psbC_p3	TGGGATTCGTTAGGACTACC	AGCCTTGAGAAACTTTGGCT	50.0	45.0	56.1	57.6	1139 bp	0.5	41.4
psbC_p4	TTGGGATTCGTTAGGACTACC	AGCCTTGAGAAACTTTGGCT	47.6	45.0	56.8	57.6	1140 bp	0.5	39.6
psbC_p5	AAAGGTTTAGTTTCGCTGAAGC	AGCCTTGAGAAACTTTGGCT	40.9	45.0	57.8	57.6	1353 bp	0.5	39.2