Hotspot

psbZ-trnS(uga)

Genome · 39484-40635 · 1152 bp

Back to Monomorphina

Pi 0.389

MarkerSeek score 82.90

Primer available yes

Label psbZ-trnS(uga)

Feature Metadata

label_name: psbZ-trnS(uga)
feature_type: igs
region: Genome
length_bp: 1152
variable_sites: 431
pi: 0.389
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnS(uga)_p1	CAGCTTGGGCAGGATTAGTT	GGATGTAGCGCAGTTTGGTA	50.0	50.0	57.9	58.0	1367 bp	0.5	58.8
psbZ-trnS(uga)_p2	TCAGGCTTCTCTTCTAGCGT	GGATGTAGCGCAGTTTGGTA	50.0	50.0	58.2	58.0	1486 bp	0.5	58.6
psbZ-trnS(uga)_p3	CAGCTTGGGCAGGATTAGTT	GGTTCGAATCCTGTCATCCC	50.0	55.0	57.9	58.0	1317 bp	0.5	58.6
psbZ-trnS(uga)_p4	TCAGGCTTCTCTTCTAGCGT	GGTTCGAATCCTGTCATCCC	50.0	55.0	58.2	58.0	1436 bp	0.5	58.4
psbZ-trnS(uga)_p5	CAGCTTGGGCAGGATTAGTT	TTTAAACGGGATGTAGCGCA	50.0	45.0	57.9	57.9	1375 bp	0.5	58.2