Hotspot

psbT-psbH

Genome · 27819-28414 · 596 bp

Back to Monomorphina

Pi 0.319
MarkerSeek score 83.00
Primer available yes
Label psbT-psbH

Feature Metadata

label_name
psbT-psbH
feature_type
igs
region
Genome
length_bp
596
variable_sites
177
pi
0.319
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbT-psbH_p1 CCTCGTGGATGGTTCACTTT CGAAACGCACAACCAATCAT 50.0 45.0 57.8 57.7 2042 bp 0.5 55.9
psbT-psbH_p2 TAGTCCTCGTGGATGGTTCA CGAAACGCACAACCAATCAT 50.0 45.0 57.8 57.7 2046 bp 0.5 55.6
psbT-psbH_p3 AAGTAGTCCTCGTGGATGGT CGAAACGCACAACCAATCAT 50.0 45.0 57.8 57.7 2049 bp 0.5 55.5
psbT-psbH_p4 AGTAGTCCTCGTGGATGGTT CGAAACGCACAACCAATCAT 50.0 45.0 57.8 57.7 2048 bp 0.5 55.5
psbT-psbH_p5 CCTCGTGGATGGTTCACTTT TGGAAAAGTTGCACCTGGAT 50.0 45.0 57.8 57.6 1444 bp 0.5 55.5