Hotspot

ndhG-ndhI

IRa · 128035-128337 · 303 bp

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Pi 0.0507

MarkerSeek score 79.70

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: IRa
length_bp: 303
variable_sites: 74
pi: 0.0507
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	TCATGTATCGGTTGGAGCAA	AATTTGTCCCAGCTCAAATA	45.0	35.0	56.9	52.7	339-414 bp	0.88	47.2
ndhG-ndhI_p2	TCATGTATCGGTTGGAGCAA	ATTTGTCCCAGCTCAAATAA	45.0	35.0	56.9	52.7	338-413 bp	0.88	47.2
ndhG-ndhI_p3	TCATGTATCGGTTGGAGCAA	AATTTGTCCCAGCTCAAATAA	45.0	33.3	56.9	53.6	339-414 bp	0.88	47.2
ndhG-ndhI_p4	ATCATGTATCGGTTGGAGCA	AATTTGTCCCAGCTCAAATA	45.0	35.0	56.7	52.7	340-415 bp	0.8	44.0
ndhG-ndhI_p5	ATCATGTATCGGTTGGAGCA	ATTTGTCCCAGCTCAAATAA	45.0	35.0	56.7	52.7	339-414 bp	0.8	44.0