Hotspot

psaC-ndhE

SSC · 126773-127015 · 243 bp

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Pi 0.0274

MarkerSeek score 76.50

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 243
variable_sites: 34
pi: 0.0274
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GAACGCATTGAGTACACCCT	AAGCAGCTATTGGATTGGCT	50.0	45.0	57.9	57.6	364-388 bp	1.0	87.6
psaC-ndhE_p2	ACATCCGTCCCAAGGTATCA	AAGCAGCTATTGGATTGGCT	50.0	45.0	58.1	57.6	411-435 bp	1.0	87.3
psaC-ndhE_p3	GAACGCATTGAGTACACCCT	GAAGCAGCTATTGGATTGGC	50.0	50.0	57.9	57.2	365-389 bp	1.0	84.9
psaC-ndhE_p4	TCGAACGCATTGAGTACACC	AAGCAGCTATTGGATTGGCT	50.0	45.0	58.3	57.6	366-390 bp	0.92	81.9
psaC-ndhE_p5	TTTCCAATACATCCGTGGGG	AAGCAGCTATTGGATTGGCT	50.0	45.0	57.6	57.6	391-415 bp	0.92	80.6