Hotspot

rpl32-trnL-UAG

SSC · 122668-123441 · 774 bp

Back to Monnina

Pi 0.044
MarkerSeek score 81.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
774
variable_sites
162
pi
0.044
unique_haplotype_count
25
primer_available
yes
alignment_reliability
0.99
markerseek_score
81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 ATATGGGAACGGGAAGTGGA AGCAAAGCCCTGGGTAAAAA 50.0 45.0 58.1 57.9 1528-1996 bp 1.0 90.3
rpl32-trnL-UAG_p2 ATATGGGAACGGGAAGTGGA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.1 57.9 1055-1524 bp 1.0 90.1
rpl32-trnL-UAG_p3 GGAACGGGAAGTGGAATGAA AGCAAAGCCCTGGGTAAAAA 50.0 45.0 57.8 57.9 1523-1991 bp 1.0 89.7
rpl32-trnL-UAG_p4 ACGGGAAGTGGAATGAAAGG AGCAAAGCCCTGGGTAAAAA 50.0 45.0 57.8 57.9 1520-1988 bp 1.0 89.6
rpl32-trnL-UAG_p5 CGGGAAGTGGAATGAAAGGT AGCAAAGCCCTGGGTAAAAA 50.0 45.0 57.8 57.9 1519-1987 bp 1.0 89.6