Hotspot

ndhI-ndhF

IRb · 119570-119815 · 246 bp

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Pi 0.0494

MarkerSeek score 77.60

Primer available yes

Label ndhI-ndhF

Feature Metadata

label_name: ndhI-ndhF
feature_type: igs
region: IRb
length_bp: 246
variable_sites: 62
pi: 0.0494
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhF_p1	TTGCTTTGGGACGTTTACCA	TATGGGTGTTGGGCGAATTT	45.0	45.0	57.9	57.8	389-538 bp	1.0	89.8
ndhI-ndhF_p2	AACAAACAGTACGAGCTGCA	TATGGGTGTTGGGCGAATTT	45.0	45.0	58.1	57.8	770-919 bp	1.0	89.3
ndhI-ndhF_p3	ACAAACAGTACGAGCTGCAA	TATGGGTGTTGGGCGAATTT	45.0	45.0	58.1	57.8	769-918 bp	1.0	89.3
ndhI-ndhF_p4	ACAAACAGTACGAGCTGCAA	GGTGTTGGGCGAATTTCTTC	45.0	50.0	58.1	57.7	765-914 bp	1.0	88.4
ndhI-ndhF_p5	AACAAACAGTACGAGCTGCA	GGTGTTGGGCGAATTTCTTC	45.0	50.0	58.1	57.7	766-915 bp	1.0	88.3