Hotspot

atpH-atpI

LSC · 22170-23534 · 1365 bp

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Pi 0.0174

MarkerSeek score 84.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1365
variable_sites: 139
pi: 0.0174
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	AAGCGGCATTCAAGCTCTTA	45.0	45.0	58.0	57.9	1324-1473 bp	1.0	90.5
atpH-atpI_p2	AGCGGCAGAAATCAATGGAT	AAGCGGCATTCAAGCTCTTA	45.0	45.0	57.9	57.9	1316-1465 bp	1.0	90.3
atpH-atpI_p3	GATCCCTTCTACAGCTTGGC	AAGCGGCATTCAAGCTCTTA	55.0	45.0	58.0	57.9	1409-1558 bp	1.0	90.2
atpH-atpI_p4	AAGCGGCAGAAATCAATGGA	AAGCGGCATTCAAGCTCTTA	45.0	45.0	58.2	57.9	1317-1466 bp	1.0	89.6
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	AAGCGGCATTCAAGCTCTTA	45.0	45.0	58.2	57.9	1325-1474 bp	1.0	89.3