Hotspot

trnR-UCU-atpA

LSC · 18118-18578 · 461 bp

Back to Monnina

Pi 0.0205

MarkerSeek score 74.70

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 461
variable_sites: 56
pi: 0.0205
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	CTACCAAGACATTCACCGGG	47.6	55.0	57.3	58.0	428-597 bp	1.0	78.2
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	TTCAGGAAGAGATGGAACGC	47.6	50.0	57.3	57.6	380-549 bp	1.0	75.4
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	CAGGAAGAGATGGAACGCTT	47.6	50.0	57.3	57.6	378-547 bp	1.0	75.3
trnR-UCU-atpA_p4	TGGATAGGACAGAGGTCTTCT	CTACCAAGACATTCACCGGG	47.6	55.0	56.9	58.0	463-632 bp	1.0	75.0
trnR-UCU-atpA_p5	GGTTCAAATCCTATTGGACGC	TCAGGAAGAGATGGAACGCT	47.6	50.0	57.3	58.4	379-548 bp	1.0	75.0