Hotspot

ycf1

SSC · 120256-125403 · 5148 bp

Back to Monanthes

Pi 0.0105

MarkerSeek score 48.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5148
variable_sites: 107
pi: 0.0105
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TACCAACCCCGACCATCTAA	TTTTGGTTCACCGCAAAACC	50.0	45.0	57.7	58.0	1030-1039 bp	1.0	81.2
ycf1_p2	CCAACCCCGACCATCTAATC	TTTTGGTTCACCGCAAAACC	55.0	45.0	57.7	58.0	1028-1037 bp	1.0	81.2
ycf1_p3	TACCAACCCCGACCATCTAA	TTCGCGAATCGTCCATTTCT	50.0	45.0	57.7	58.0	761-770 bp	1.0	81.1
ycf1_p4	CCAACCCCGACCATCTAATC	TTCGCGAATCGTCCATTTCT	55.0	45.0	57.7	58.0	759-768 bp	1.0	81.1
ycf1_p5	TACCAACCCCGACCATCTAA	TTGGCATAGATCGAAACCCG	50.0	50.0	57.7	58.1	1138-1144 bp	0.75	68.2