Hotspot

psaB

LSC · 37176-39380 · 2205 bp

Back to Monanthes

Pi 0.00234

MarkerSeek score 58.90

Primer available yes

Label psaB

Feature Metadata

label_name: psaB
feature_type: gene
region: LSC
length_bp: 2205
variable_sites: 10
pi: 0.00234
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaB_p1	TGCCATAATGGTTCAGCTCC	TACAAGGACGTGCTGTAGGA	50.0	50.0	57.9	58.1	2444 bp	1.0	79.2
psaB_p2	ATAGGACCCTTCTGCATCGA	TACAAGGACGTGCTGTAGGA	50.0	50.0	57.9	58.1	2374 bp	1.0	78.9
psaB_p3	TAGGACCCTTCTGCATCGAA	TACAAGGACGTGCTGTAGGA	50.0	50.0	58.1	58.1	2373 bp	1.0	78.5
psaB_p4	TGCCATAATGGTTCAGCTCC	AAGGACGTGCTGTAGGAGTA	50.0	50.0	57.9	57.8	2441 bp	1.0	78.2
psaB_p5	TGCCATAATGGTTCAGCTCC	AGGACGTGCTGTAGGAGTAA	50.0	50.0	57.9	57.8	2440 bp	1.0	78.2