Hotspot

clpP

LSC · 67718-69753 · 2036 bp

Back to Monanthes

Pi 0.00737

MarkerSeek score 48.70

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2036
variable_sites: 29
pi: 0.00737
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	ACGGTTGTGAAATCCTTGCT	TCCTAAACGCGTCATGAAGG	45.0	50.0	58.0	58.0	2701-2709 bp	1.0	81.4
clpP_p2	ACGGTTGTGAAATCCTTGCT	GATTCGTTACAGTTCCGCCT	45.0	50.0	58.0	58.0	2753-2761 bp	1.0	81.4
clpP_p3	CTGAACGAGTCGCACATACA	TCCTAAACGCGTCATGAAGG	50.0	50.0	58.0	58.0	2969-2977 bp	1.0	81.4
clpP_p4	CAAGCCCATGATCTGAACGA	TCCTAAACGCGTCATGAAGG	50.0	50.0	58.0	58.0	2981-2989 bp	1.0	81.4
clpP_p5	ACGGTTGTGAAATCCTTGCT	CCGCCTGTAATACTCCAACC	45.0	55.0	58.0	58.1	2739-2747 bp	1.0	81.0