Hotspot

trnR-ACG-trnN-GUU

IRb · 105553-106161 · 609 bp

Back to Monanthes

Pi 0.00657
MarkerSeek score 63.30
Primer available yes
Label trnR-ACG-trnN-GUU

Feature Metadata

label_name
trnR-ACG-trnN-GUU
feature_type
igs
region
IRb
length_bp
609
variable_sites
8
pi
0.00657
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-trnN-GUU_p1 ACAGATCTAGTGGAGACGGG TGGTCGTAGGTTCGAATCCT 55.0 50.0 57.9 58.2 736 bp 1.0 79.1
trnR-ACG-trnN-GUU_p2 CTCCCGGGAACAGATCTAGT TGGTCGTAGGTTCGAATCCT 55.0 50.0 57.9 58.2 745 bp 1.0 79.0
trnR-ACG-trnN-GUU_p3 ACAGATCTAGTGGAGACGGG CGATTGGTCGTAGGTTCGAA 55.0 50.0 57.9 57.7 740 bp 1.0 78.2
trnR-ACG-trnN-GUU_p4 CTCCCGGGAACAGATCTAGT CGATTGGTCGTAGGTTCGAA 55.0 50.0 57.9 57.7 749 bp 1.0 78.1
trnR-ACG-trnN-GUU_p5 ACAGATCTAGTGGAGACGGG TTCCTCAGTAGCTCAGTGGT 55.0 50.0 57.9 57.7 779 bp 1.0 77.9