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psbZ-trnG-GCC

LSC · 35633-36324 · 692 bp

Back to Monanthes

Pi 0.00747

MarkerSeek score 59.40

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 692
variable_sites: 10
pi: 0.00747
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTGCTTCTCCTGATGGTTGG	AATCGAACCCGCATCTTCTC	50.0	50.0	57.8	58.1	758-892 bp	1.0	82.8
psbZ-trnG-GCC_p2	TTGCTTCTCCTGATGGTTGG	GAATCGAACCCGCATCTTCT	50.0	50.0	57.8	58.1	759-893 bp	1.0	82.8
psbZ-trnG-GCC_p3	GCTTCTCCTGATGGTTGGTC	AATCGAACCCGCATCTTCTC	55.0	50.0	58.3	58.1	756-890 bp	1.0	82.4
psbZ-trnG-GCC_p4	GCTTCTCCTGATGGTTGGTC	GAATCGAACCCGCATCTTCT	55.0	50.0	58.3	58.1	757-891 bp	1.0	82.4
psbZ-trnG-GCC_p5	CTCCTGATGGTTGGTCAAGC	AATCGAACCCGCATCTTCTC	55.0	50.0	58.5	58.1	752-886 bp	1.0	80.1