Hotspot

petN-psbM

LSC · 28520-29313 · 794 bp

Back to Monanthes

Pi 0.0136

MarkerSeek score 55.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 794
variable_sites: 21
pi: 0.0136
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTGTTCATTCT	45.0	42.9	58.0	57.6	942-962 bp	1.0	71.9
petN-psbM_p2	CACTAGTAGTGTGGGGACGA	TGCTACTGCACTGTTCATTCT	55.0	42.9	58.2	57.6	898-918 bp	1.0	70.7
petN-psbM_p3	TCACTAGTAGTGTGGGGACG	TGCTACTGCACTGTTCATTCT	55.0	42.9	58.2	57.6	899-919 bp	1.0	70.7
petN-psbM_p4	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.8	57.6	948-968 bp	1.0	70.4
petN-psbM_p5	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTGTTCATTC	45.0	45.0	58.0	56.1	942-962 bp	1.0	67.7