Hotspot

rpoB-trnC-GCA

LSC · 26626-27550 · 925 bp

Back to Monanthes

Pi 0.0121

MarkerSeek score 59.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 925
variable_sites: 22
pi: 0.0121
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GTGGACCTTCCCTCATTTCC	CCGGATTTGAACTGGGGAAA	55.0	50.0	57.9	58.1	1005-1019 bp	1.0	81.2
rpoB-trnC-GCA_p2	GTGGACCTTCCCTCATTTCC	ATCGGGCGAACATAATACGG	55.0	50.0	57.9	57.9	1056-1070 bp	1.0	81.1
rpoB-trnC-GCA_p3	GTGGACCTTCCCTCATTTCC	CGGGCGAACATAATACGGAA	55.0	50.0	57.9	58.1	1054-1068 bp	1.0	80.6
rpoB-trnC-GCA_p4	TGGACCTTCCCTCATTTCCA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	1004-1018 bp	1.0	80.3
rpoB-trnC-GCA_p5	TGGACCTTCCCTCATTTCCA	ATCGGGCGAACATAATACGG	50.0	50.0	58.2	57.9	1055-1069 bp	1.0	80.2