Hotspot

trnS-GCU-trnR-UCU

LSC · 8625-10128 · 1504 bp

Back to Monanthes

Pi 0.0103

MarkerSeek score 60.10

Primer available yes

Label trnS-GCU-trnR-UCU

Feature Metadata

label_name: trnS-GCU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 1504
variable_sites: 30
pi: 0.0103
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	ATTAGCAATCCGGCGCTTTA	AGGTTTAGAAGACCTATGTCCT	45.0	40.9	58.0	55.6	1586-1615 bp	1.0	46.5
trnS-GCU-trnR-UCU_p2	ATTAGCAATCCGGCGCTTTA	AGAAGACCTATGTCCTATCCA	45.0	42.9	58.0	54.6	1580-1609 bp	1.0	46.3
trnS-GCU-trnR-UCU_p3	CGAATCGATCGTACAACGGA	AGGTTTAGAAGACCTATGTCCT	50.0	40.9	57.9	55.6	1605-1634 bp	1.0	46.1
trnS-GCU-trnR-UCU_p4	ATTAGCAATCCGGCGCTTTA	ACCAAAGGTTTAGAAGACCTATGT	45.0	37.5	58.0	57.6	1591-1620 bp	1.0	45.9
trnS-GCU-trnR-UCU_p5	CGAATCGATCGTACAACGGA	AGAAGACCTATGTCCTATCCA	50.0	42.9	57.9	54.6	1599-1628 bp	1.0	45.8