Hotspot

trnH-GUG-psbA

LSC · 72-331 · 260 bp

Back to Monanthes

Pi 0.0295

MarkerSeek score 72.00

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 260
variable_sites: 15
pi: 0.0295
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	GTAACGCTCACAACTTCCCT	55.0	50.0	58.1	57.8	334-345 bp	1.0	84.7
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	GTAACGCTCACAACTTCCCT	50.0	50.0	57.9	57.8	348-359 bp	1.0	84.4
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	GTAACGCTCACAACTTCCCT	50.0	50.0	57.9	57.8	347-358 bp	1.0	84.4
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	GTAACGCTCACAACTTCCCT	50.0	50.0	57.9	57.8	349-360 bp	1.0	84.4
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	TGAACGTAACGCTCACAACT	55.0	45.0	58.1	57.8	339-350 bp	1.0	84.2