Hotspot

accD

LSC · 60614-62146 · 1533 bp

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Pi 0.0307

MarkerSeek score 68.10

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1533
variable_sites: 65
pi: 0.0307
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	CTGGATCCGCAATTAAGCCT	AATCCGATCCAACAACAGGG	50.0	50.0	58.0	57.9	2221-2457 bp	1.0	85.6
accD_p2	CTGGATCCGCAATTAAGCCT	CCGATCCAACAACAGGGATT	50.0	50.0	58.0	57.9	2218-2454 bp	1.0	85.6
accD_p3	CGTAGCGTCGTTTCAGATCA	AATCCGATCCAACAACAGGG	50.0	50.0	58.1	57.9	2158-2394 bp	1.0	85.0
accD_p4	CGTAGCGTCGTTTCAGATCA	CCGATCCAACAACAGGGATT	50.0	50.0	58.1	57.9	2155-2391 bp	1.0	85.0
accD_p5	TTGTTATGCTGGATCCGCAA	CCGATCCAACAACAGGGATT	45.0	50.0	57.9	57.9	2226-2462 bp	1.0	84.9