Hotspot

ycf1-trnN-GUU

IRa · 132299-134185 · 1887 bp

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Pi 0.0124

MarkerSeek score 51.50

Primer available yes

Label ycf1-trnN-GUU

Feature Metadata

label_name: ycf1-trnN-GUU
feature_type: igs
region: IRa
length_bp: 1887
variable_sites: 35
pi: 0.0124
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN-GUU_p1	ACGTTTGGCTTTTCGTGAAC	CGGGTTACCCACTCTTAACG	45.0	55.0	57.8	58.0	2357-2414 bp	1.0	80.6
ycf1-trnN-GUU_p2	TTACGTCTTTGAGCACGCAT	CGGGTTACCCACTCTTAACG	45.0	55.0	58.2	58.0	2521-2593 bp	1.0	80.5
ycf1-trnN-GUU_p3	ACGTTTGGCTTTTCGTGAAC	TCGTTAATGGGTTAAGGGCG	45.0	50.0	57.8	58.0	2768-2821 bp	1.0	80.2
ycf1-trnN-GUU_p4	ACGTTTGGCTTTTCGTGAAC	GGCAGAATAGCAGAGCAAGT	45.0	50.0	57.8	58.0	2656-2709 bp	1.0	80.2
ycf1-trnN-GUU_p5	ACGTTTGGCTTTTCGTGAAC	CCCGTAATTCTTCCTCAGCC	45.0	55.0	57.8	58.0	2683-2736 bp	1.0	80.1