Hotspot

rpl32-trnL-UAG

SSC · 117250-118570 · 1321 bp

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Pi 0.0382
MarkerSeek score 74.80
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1321
variable_sites
65
pi
0.0382
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GCAAAATCTCTTTCAACGGGG AGCGTGTCTACCGATTTCAC 47.6 50.0 58.1 58.0 1160-1410 bp 1.0 79.5
rpl32-trnL-UAG_p2 GCAAAATCTCTTTCAACGGGG CAGCGTGTCTACCGATTTCA 47.6 50.0 58.1 58.0 1161-1411 bp 1.0 79.5
rpl32-trnL-UAG_p3 TCAACGGGGAATTCACAAAGT AGCGTGTCTACCGATTTCAC 42.9 50.0 58.1 58.0 1148-1398 bp 1.0 79.4
rpl32-trnL-UAG_p4 TCAACGGGGAATTCACAAAGT CAGCGTGTCTACCGATTTCA 42.9 50.0 58.1 58.0 1149-1399 bp 1.0 79.4
rpl32-trnL-UAG_p5 GCAAAATCTCTTTCAACGGGG TCCTAAGAGCAGCGTGTCTA 47.6 50.0 58.1 57.9 1170-1420 bp 1.0 78.5