Hotspot

petA-psbJ

LSC · 66258-67258 · 1001 bp

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Pi 0.0207

MarkerSeek score 73.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1001
variable_sites: 30
pi: 0.0207
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	GCCGAGACTACTGGAAGGAT	50.0	55.0	58.0	58.6	1220-1240 bp	1.0	78.6
petA-psbJ_p2	CATTACGTGTCCAAGGCCTT	GCCGAGACTACTGGAAGGAT	50.0	55.0	58.2	58.6	1217-1237 bp	1.0	77.5
petA-psbJ_p3	TTACGTGTCCAAGGCCTTTT	GCCGAGACTACTGGAAGGAT	45.0	55.0	57.6	58.6	1215-1235 bp	1.0	76.2
petA-psbJ_p4	TACGTGTCCAAGGCCTTTTG	GCCGAGACTACTGGAAGGAT	50.0	55.0	58.4	58.6	1214-1234 bp	1.0	75.8
petA-psbJ_p5	TGTCCAAGGCCTTTTGTTCT	GCCGAGACTACTGGAAGGAT	45.0	55.0	57.5	58.6	1210-1230 bp	1.0	75.4