Hotspot

trnM-CAU-atpE

LSC · 55640-55803 · 164 bp

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Pi 0.0695
MarkerSeek score 75.10
Primer available yes
Label trnM-CAU-atpE

Feature Metadata

label_name
trnM-CAU-atpE
feature_type
igs
region
LSC
length_bp
164
variable_sites
13
pi
0.0695
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-atpE_p1 TGGACCGAACCTTTTGTCAG CTCTCAGACGAGCTAGGACA 50.0 55.0 58.0 58.0 620-629 bp 1.0 85.1
trnM-CAU-atpE_p2 GCTCCATGTGCTCTGATTCA CTCTCAGACGAGCTAGGACA 50.0 55.0 58.0 58.0 953-954 bp 1.0 84.1
trnM-CAU-atpE_p3 GCTCCATGTGCTCTGATTCA GGAAATACGTTGGCCTAGCA 50.0 50.0 58.0 58.0 1468-1469 bp 1.0 82.6
trnM-CAU-atpE_p4 GCTCCATGTGCTCTGATTCA GCACGAGCACGAAAAATTGA 50.0 45.0 58.0 58.0 1541-1542 bp 1.0 82.5
trnM-CAU-atpE_p5 TGGACCGAACCTTTTGTCAG GCACGAGCACGAAAAATTGA 50.0 45.0 58.0 58.0 1208-1217 bp 1.0 82.5