Hotspot

psbM-trnD-GUC

LSC · 31384-32494 · 1111 bp

Back to Momordica

Pi 0.0287

MarkerSeek score 72.20

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1111
variable_sites: 44
pi: 0.0287
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGGCGGTAGGAACTAGAATGA	TTCAATTGGTCAGAGCACCG	47.6	50.0	58.0	58.5	1158-1242 bp	1.0	74.9
psbM-trnD-GUC_p2	AGGCGGTAGGAACTAGAATGA	GTTCAATTGGTCAGAGCACC	47.6	50.0	58.0	57.0	1159-1243 bp	1.0	70.7
psbM-trnD-GUC_p3	GGCGGTAGGAACTAGAATGA	TTCAATTGGTCAGAGCACCG	50.0	50.0	56.4	58.5	1157-1241 bp	1.0	70.6
psbM-trnD-GUC_p4	AGGCGGTAGGAACTAGAATG	TTCAATTGGTCAGAGCACCG	50.0	50.0	56.4	58.5	1158-1242 bp	1.0	70.5
psbM-trnD-GUC_p5	AGGCGGTAGGAACTAGAATGA	TCAATTGGTCAGAGCACCG	47.6	52.6	58.0	57.8	1157-1241 bp	1.0	68.8