Hotspot

atpH-atpI

LSC · 14987-16132 · 1146 bp

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Pi 0.0149

MarkerSeek score 72.40

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1146
variable_sites: 25
pi: 0.0149
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1208-1233 bp	1.0	83.1
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	45.0	55.0	58.0	57.8	1178-1203 bp	1.0	82.9
atpH-atpI_p3	GATCCCTTCTACAGCTTGGC	TTTTGCAACTTTAGCTGCGG	55.0	45.0	58.0	57.9	1293-1318 bp	1.0	82.8
atpH-atpI_p4	GATCCCTTCTACAGCTTGGC	CGAATCCATGGAGGGTCATC	55.0	55.0	58.0	57.8	1263-1288 bp	1.0	82.5
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.2	57.9	1209-1234 bp	1.0	81.9