Hotspot

trnS-GCU-trnR-UCU

LSC · 9144-10980 · 1837 bp

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Pi 0.0248
MarkerSeek score 69.70
Primer available yes
Label trnS-GCU-trnR-UCU

Feature Metadata

label_name
trnS-GCU-trnR-UCU
feature_type
igs
region
LSC
length_bp
1837
variable_sites
67
pi
0.0248
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnR-UCU_p1 CAATCCGACGCTTTAGTCCA AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 1895-1930 bp 1.0 69.3
trnS-GCU-trnR-UCU_p2 AATCCGACGCTTTAGTCCAC AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 1894-1929 bp 1.0 69.3
trnS-GCU-trnR-UCU_p3 CAATCCGACGCTTTAGTCCA AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 1901-1936 bp 1.0 68.6
trnS-GCU-trnR-UCU_p4 AATCCGACGCTTTAGTCCAC AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 1900-1935 bp 1.0 68.6
trnS-GCU-trnR-UCU_p5 ACGGAAAGAGAGGGATTCGA AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.2 56.9 1949-1984 bp 1.0 68.0