Hotspot

psbI-trnS-GCU

LSC · 8951-9055 · 105 bp

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Pi 0.0698
MarkerSeek score 79.20
Primer available yes
Label psbI-trnS-GCU

Feature Metadata

label_name
psbI-trnS-GCU
feature_type
igs
region
LSC
length_bp
105
variable_sites
11
pi
0.0698
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS-GCU_p1 TCCTATCTAATGACCCGGGAC TGGACTAAAGCGTCGGATTG 52.4 50.0 58.1 58.0 222-226 bp 1.0 75.0
psbI-trnS-GCU_p2 TCCTATCTAATGACCCGGGAC GTGGACTAAAGCGTCGGATT 52.4 50.0 58.1 58.0 223-227 bp 1.0 75.0
psbI-trnS-GCU_p3 TCCTATCTAATGACCCGGGA TGGACTAAAGCGTCGGATTG 50.0 50.0 56.6 58.0 222-226 bp 1.0 72.8
psbI-trnS-GCU_p4 TCCTATCTAATGACCCGGGA GTGGACTAAAGCGTCGGATT 50.0 50.0 56.6 58.0 223-227 bp 1.0 72.8
psbI-trnS-GCU_p5 CCTATCTAATGACCCGGGAC TGGACTAAAGCGTCGGATTG 55.0 50.0 56.6 58.0 221-225 bp 1.0 72.5