Hotspot

ycf66

Genome · 530-1528 · 999 bp

Back to Moerckia

Pi 0.0251

MarkerSeek score 59.70

Primer available yes

Label ycf66

Feature Metadata

label_name: ycf66
feature_type: gene
region: Genome
length_bp: 999
variable_sites: 25
pi: 0.0251
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf66_p1	ATTCCATTGGCAGCACAGAA	AAGTGGTAAGGCAGAGGACT	45.0	50.0	58.1	58.0	2007-2373 bp	1.0	82.9
ycf66_p2	ATTCCATTGGCAGCACAGAA	CGCTTCGCCGACAAGATATA	45.0	50.0	58.1	58.0	1953-2319 bp	1.0	82.9
ycf66_p3	TGAAACGGTATACACGCGAG	AAGTGGTAAGGCAGAGGACT	50.0	50.0	58.1	58.0	2126-2492 bp	1.0	82.5
ycf66_p4	GGACTACGACATAGGGGACA	AAGTGGTAAGGCAGAGGACT	55.0	50.0	57.9	58.0	1677 bp	0.5	59.2
ycf66_p5	GGACTACGACATAGGGGACA	CGCTTCGCCGACAAGATATA	55.0	50.0	57.9	58.0	1623 bp	0.5	59.1