Hotspot

rps12-trnV-GAC

Genome · 85678-86386 · 709 bp

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Pi 0.0527

MarkerSeek score 80.30

Primer available yes

Label rps12-trnV-GAC

Feature Metadata

label_name: rps12-trnV-GAC
feature_type: igs
region: Genome
length_bp: 709
variable_sites: 37
pi: 0.0527
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-trnV-GAC_p1	AGGGCGGAATTTGGTTTCTT	CTCGAACTGACGACTTCCAC	45.0	55.0	58.0	58.3	802-848 bp	1.0	81.7
rps12-trnV-GAC_p2	CCTAACTCGAGCCACTTTCC	CTCGAACTGACGACTTCCAC	55.0	55.0	58.0	58.3	824-870 bp	1.0	81.6
rps12-trnV-GAC_p3	GCGGAATTTGGTTTCTTCGG	CTCGAACTGACGACTTCCAC	50.0	55.0	58.0	58.3	799-845 bp	1.0	81.6
rps12-trnV-GAC_p4	GGCGGAATTTGGTTTCTTCG	CTCGAACTGACGACTTCCAC	50.0	55.0	58.0	58.3	800-846 bp	1.0	81.6
rps12-trnV-GAC_p5	ACCTGGGATATATGCGGTGA	CTCGAACTGACGACTTCCAC	50.0	55.0	58.0	58.3	863-909 bp	1.0	81.3