Hotspot

trnW-CCA-trnP-UGG

Genome · 64429-64479 · 51 bp

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Pi 0.0588

MarkerSeek score 74.30

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: Genome
length_bp: 51
variable_sites: 3
pi: 0.0588
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	TGCGTTCTACCGTACTGAAC	GGTTCGAATCCTGTCATCCC	50.0	55.0	57.7	58.0	101 bp	1.0	79.3
trnW-CCA-trnP-UGG_p2	TGCGTTCTACCGTACTGAAC	GGATGTAGCGCAGTTTGGTA	50.0	50.0	57.7	58.0	151 bp	1.0	78.7
trnW-CCA-trnP-UGG_p3	GTTTTGGAGACCTGCGTTCT	GGTTCGAATCCTGTCATCCC	50.0	55.0	58.4	58.0	113 bp	1.0	78.5
trnW-CCA-trnP-UGG_p4	GTTTTGGAGACCTGCGTTCT	GGATGTAGCGCAGTTTGGTA	50.0	50.0	58.4	58.0	163 bp	1.0	78.1
trnW-CCA-trnP-UGG_p5	TTTGGAGACCTGCGTTCTAC	GGATGTAGCGCAGTTTGGTA	50.0	50.0	57.5	58.0	161 bp	1.0	77.7