Hotspot

cemA-petA

Genome · 61395-61587 · 193 bp

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Pi 0.0582

MarkerSeek score 75.70

Primer available yes

Label cemA-petA

Feature Metadata

label_name: cemA-petA
feature_type: igs
region: Genome
length_bp: 193
variable_sites: 11
pi: 0.0582
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA-petA_p1	CCTTCCATTGTGGCAACCTA	TGCGAAACAGATGACCGAAT	50.0	45.0	57.8	57.9	332-333 bp	1.0	81.1
cemA-petA_p2	CCTTCCATTGTGGCAACCTA	CAAAAATCGGGTATGCCTGC	50.0	50.0	57.8	57.8	349-350 bp	1.0	80.1
cemA-petA_p3	CCTTCCATTGTGGCAACCTA	GCAAAAATCGGGTATGCCTG	50.0	50.0	57.8	57.8	350-351 bp	1.0	80.0
cemA-petA_p4	ATATCCCCTTCCATTGTGGC	TGCGAAACAGATGACCGAAT	50.0	45.0	57.3	57.9	338-339 bp	1.0	77.3
cemA-petA_p5	TATCCCCTTCCATTGTGGCA	TGCGAAACAGATGACCGAAT	50.0	45.0	58.7	57.9	337-338 bp	1.0	77.2