Hotspot

trnF-GAA-ndhJ

Genome · 50035-50163 · 129 bp

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Pi 0.116

MarkerSeek score 73.70

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: Genome
length_bp: 129
variable_sites: 15
pi: 0.116
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	AAACTCGCGGCGGTTATAAT	ACGAGTTGCAGGATTCGTAC	45.0	50.0	58.1	58.0	249-291 bp	1.0	84.2
trnF-GAA-ndhJ_p2	AACTCGCGGCGGTTATAATT	ACGAGTTGCAGGATTCGTAC	45.0	50.0	58.1	58.0	248-290 bp	1.0	84.2
trnF-GAA-ndhJ_p3	TCGTTCGGATGATTCGCAAT	ACGAGTTGCAGGATTCGTAC	45.0	50.0	58.1	58.0	880-924 bp	1.0	82.1
trnF-GAA-ndhJ_p4	TTGATCCGATCGAGCTTTGG	ACGAGTTGCAGGATTCGTAC	50.0	50.0	58.1	58.0	727-774 bp	1.0	81.8
trnF-GAA-ndhJ_p5	TCGTGTCACCAGTTCGAATC	ACGAGTTGCAGGATTCGTAC	50.0	50.0	57.9	58.0	187 bp	0.5	59.5