Hotspot

trnE-UUC-CysA

Genome · 34785-35114 · 330 bp

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Pi 0.0484

MarkerSeek score 76.60

Primer available yes

Label trnE-UUC-CysA

Feature Metadata

label_name: trnE-UUC-CysA
feature_type: igs
region: Genome
length_bp: 330
variable_sites: 15
pi: 0.0484
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-CysA_p1	CCGTCGTCTCCTTGAAAGAG	ACTCACACGATCCAGAATTTGT	55.0	40.9	58.0	58.0	446-505 bp	1.0	69.7
trnE-UUC-CysA_p2	CCGTCGTCTCCTTGAAAGAG	ACTTCGGAAACAAGAATACCCA	55.0	40.9	58.0	57.6	403-462 bp	1.0	67.5
trnE-UUC-CysA_p3	GAAAGAGAGATGTCCTGGGC	ACTCACACGATCCAGAATTTGT	55.0	40.9	57.7	58.0	433-492 bp	1.0	67.4
trnE-UUC-CysA_p4	GTTTCTGTCTTACCCCCAGG	ACTCACACGATCCAGAATTTGT	55.0	40.9	57.5	58.0	479-538 bp	1.0	65.5
trnE-UUC-CysA_p5	CCGTCGTCTCCTTGAAAGAG	TGACTTGGATACTTCGGAAACA	55.0	40.9	58.0	57.4	413-472 bp	1.0	65.3