Hotspot

trnD-GUC-trnY-GUA

Genome · 34374-34559 · 186 bp

Back to Moerckia

Pi 0.0383

MarkerSeek score 73.70

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: Genome
length_bp: 186
variable_sites: 7
pi: 0.0383
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	TTGACAGGGCGGTACTCTAA	CTCGAGTGGTTAATGGGGAC	50.0	55.0	58.1	57.7	296-298 bp	1.0	79.6
trnD-GUC-trnY-GUA_p2	TTGACAGGGCGGTACTCTAA	ATGCTCGAGTGGTTAATGGG	50.0	50.0	58.1	57.7	299-301 bp	1.0	79.4
trnD-GUC-trnY-GUA_p3	TTGACAGGGCGGTACTCTAA	GTTTCTATCTGGGCTGGGTC	50.0	55.0	58.1	57.7	322 bp	0.5	56.8
trnD-GUC-trnY-GUA_p4	TTGACAGGGCGGTACTCTAA	CACGTTTCTATCTGGGCTGG	50.0	55.0	58.1	58.3	325 bp	0.5	56.7
trnD-GUC-trnY-GUA_p5	TTGACAGGGCGGTACTCTAA	ACACGTTTCTATCTGGGCTG	50.0	50.0	58.1	57.6	326 bp	0.5	56.3