Hotspot

trnR-UCU-trnG-GCC

Genome · 18388-18471 · 84 bp

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Pi 0.0714

MarkerSeek score 76.70

Primer available yes

Label trnR-UCU-trnG-GCC

Feature Metadata

label_name: trnR-UCU-trnG-GCC
feature_type: igs
region: Genome
length_bp: 84
variable_sites: 6
pi: 0.0714
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-trnG-GCC_p1	AGGGTACAGCTACGTCCAAT	ACCCTTAGCCTTCCAAGCTA	50.0	50.0	58.1	58.0	215-227 bp	1.0	80.8
trnR-UCU-trnG-GCC_p2	AGGGTACAGCTACGTCCAAT	AACGAAAAGTACGATGCCGA	50.0	45.0	58.1	57.9	240-252 bp	1.0	80.3
trnR-UCU-trnG-GCC_p3	AGGGTACAGCTACGTCCAAT	CAACGAAAAGTACGATGCCG	50.0	50.0	58.1	57.9	241-253 bp	1.0	79.7
trnR-UCU-trnG-GCC_p4	AGGGTACAGCTACGTCCAAT	ACGAAAAGTACGATGCCGAT	50.0	45.0	58.1	57.7	239-251 bp	1.0	78.5
trnR-UCU-trnG-GCC_p5	AGGGTACAGCTACGTCCAAT	TTTCCACGTTCGGTTCTGAA	50.0	45.0	58.1	57.7	272-284 bp	1.0	78.1