Hotspot

petN-trnC-GCA

Genome · 1718-2092 · 375 bp

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Pi 0.0749

MarkerSeek score 77.60

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: Genome
length_bp: 375
variable_sites: 26
pi: 0.0749
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	TCGAAGCACCCCAAGCTATA	AAGTGGTAAGGCAGAGGACT	50.0	50.0	58.5	58.0	466-815 bp	1.0	82.4
petN-trnC-GCA_p2	CGAAGCACCCCAAGCTATAT	AAGTGGTAAGGCAGAGGACT	50.0	50.0	57.4	58.0	465-814 bp	1.0	82.1
petN-trnC-GCA_p3	TCGAAGCACCCCAAGCTATA	CAAGTGGTAAGGCAGAGGAC	50.0	55.0	58.5	57.9	467-816 bp	1.0	81.9
petN-trnC-GCA_p4	TCGAAGCACCCCAAGCTATA	CAAATCCTTCATCCCCGGTT	50.0	50.0	58.5	57.9	445-794 bp	1.0	81.8
petN-trnC-GCA_p5	CGAAGCACCCCAAGCTATAT	CAAGTGGTAAGGCAGAGGAC	50.0	55.0	57.4	57.9	466-815 bp	1.0	81.5