Hotspot

ycf66-petN

Genome · 1529-1618 · 90 bp

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Pi 0.0674

MarkerSeek score 72.10

Primer available yes

Label ycf66-petN

Feature Metadata

label_name: ycf66-petN
feature_type: igs
region: Genome
length_bp: 90
variable_sites: 6
pi: 0.0674
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf66-petN_p1	TGTTACGGAGAGAAAGAACGA	TATGGGGAAGAAGCGGTTTG	42.9	50.0	56.7	57.9	143-151 bp	1.0	64.7
ycf66-petN_p2	TGTTACGGAGAGAAAGAACGA	GTATGGGGAAGAAGCGGTTT	42.9	50.0	56.7	57.9	144-152 bp	1.0	64.6
ycf66-petN_p3	TTGTTACGGAGAGAAAGAACGA	TATGGGGAAGAAGCGGTTTG	40.9	50.0	57.4	57.9	144-152 bp	1.0	62.2
ycf66-petN_p4	TTGTTACGGAGAGAAAGAACGA	GTATGGGGAAGAAGCGGTTT	40.9	50.0	57.4	57.9	145-153 bp	1.0	62.2
ycf66-petN_p5	TGTTACGGAGAGAAAGAACGA	GTTGTATGGGGAAGAAGCGG	42.9	55.0	56.7	58.6	147-155 bp	1.0	60.6